Observations on the feeding behaviour of parasitic third-stage hookworm larvae

Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm).

A technique for the cryopreservation of third stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed third-stage larvae in 80% (v/v) ethylene glycol as a cryoprotectant for 30 s at 0 degrees C, prior to transfer into liquid nitrogen. The survival rate, as assessed by motility, was 37.6% after 30 d cryopreservation. A sheep infected percutaneously with 700 live cry...

Identification of abundant mRNAs from the third stage larvae of the parasitic nematode, ostertagia ostertagi.

Reverse transcriptase-PCR (RT-PCR) was carried out on total RNA prepared from the third-stage larvae (L3) of Ostertagia ostertagi in order to clone and characterize the major transcripts expressed in this larval stage, as an initial investigation of arrested larval development in the parasite. Distinct bands were visible on an agarose gel and four of these were cloned and sequenced. Three of th...

Studies on hookworm larvae. V. Further observations of infective larvae in migration towards vegetables.

In the report III (1954) of this series, I reported the experimental results of some investigations carried out during the summer season from July to August 1952-1953. Through these former investigations, I came to understand many points of the migration towards vegetables of the hookworm larvae. However it was unexpected that most of them were easily destroyed by the sun's rays after migration...

Some observations on the feeding of the guinea pig

Observations on the Treatment of Hookworm Disease

as headache, giddiness, nausea and vomiting, weakness, palpitation of the heart, weak pulse, were more marked in the early cases, when the dose of mag. sulph. was \ oz., than in later cases, when the dose had been increased to 1 oz. 2. It was noticed that if patients were allowed to drink copious draughts of water during treatment, vomiting was fairly frequent; hut if the water, was only given ...